目的 研究紫雪散对大鼠胶原诱导性关节炎的治疗作用及其机制研究。方法 Wistar大鼠随机分为5组，分别为对照组、模型组、吲哚美辛片（2 mg/kg）组以及紫雪散0.5、1.0 g/kg组，每组10只。建立大鼠Ⅱ型胶原诱导性关节炎模型。大鼠初次免疫后第12天开始灌胃给药，给药容量10 mL/kg，1次/d。对照组、模型组均灌胃给予同体积生理盐水。各组均持续给药3周。测量大鼠左后足踝宽度、厚度和周长，计算关节炎指数。观察大鼠踝关节病理。采用ELISA法测定血清炎症因子血小板活化因子（PAF）水平、环氧合酶-2（COX-2）活性、肿瘤坏死因子α（TNF-α）水平，采用比浊法测定血清循环免疫复合物（CIC）水平。结果 给药7 d，紫雪散组踝关节宽度、厚度显著低于模型组并能持续到给药21 d。紫雪散组滑膜细胞增生明显减轻，滑膜组织充血水肿明显减轻。与模型组比较，紫雪散组全身表现评分、关节肿胀指数和关节炎指数均显著降低（P<0.05、0.01、0.001）。与模型组比较，紫雪散0.5、1.0 g/kg组血清PAF水平显著减少，COX-2活性显著降低（P<0.001）。与模型组比较，紫雪散0.5、1.0 g/kg组血清TNF-α、CIC水平显著减少（P<0.05、0.01、0.001）。结论 紫雪散对大鼠胶原诱导性关节炎损伤具有治疗效果，其机制可能与紫雪散的抗炎和抗氧化作用有关。

Objective To investigate the therapeutic effects of Zixue Powder on collagen-induced arthritis in rats and its mechanism. Methods Wistar rats were randomly divided into control group, model group, Indometacin Tablets (2 mg/kg) group, and Zixue Powder (0.5 and 1.0 g/kg) groups, and each group had 10 mice. Type Ⅱ collagen-induced arthritis model in rats was established. Rats were ig administered from 12th day after the first immunization, and the volume was 10 mL/kg, 1 time/d. Rats in the control group and model group were given the same volume of normal saline. All animals were treated for 3 weeks. Left ankle joint width, thickness, and perimeter were determined, and arthritis indexes were calculated. Pathology of rat ankle joint was observed. The lever of PAF, COX-2, and TNF-α were determined by ELISA method, and level of circulating immune complex was determined by turbidimetry method. Results Treatment for 7 d continue to 21 d, ankle joint width and thickness of rats in the Zixue Powder groups were lower than those of rats in model group. The proliferations of synovial cells were significantly reduced in the Zixue Powder groups, and synovial tissue edema was also significantly reduced. Compared with model group, systemic manifestations score, joint swelling index, and arthritis index in the Zixue Powder group were significantly decreased (P<0.05, 0.01, and 0.001). Compared with model group, levels of serum PAF in the Zixue Powder groups were significantly reduced, activities of COX-2 were significantly decreased (P<0.01), and levels of CIC and TNF-α were significantly decreased (P<0.05, 0.01, and 0.001).Conclusions Zixue Powder has therapeutic effects on collagen-induced arthritis in rats, which may be in regard to the antiinflammatory properties and anti-oxidant activity.