目的 建立HPLC法同时测定青贯解毒颗粒中去甲氧基荚果蕨素、重楼皂苷Ⅶ、重楼皂苷Ⅱ和重楼皂苷Ⅰ。方法 采用Agilent Zorbax SB C₁₈色谱柱（250 mm×4.6 mm，5 μm）；流动相A：甲醇-乙腈（2∶1），流动相B：水，采用梯度洗脱；0～32 min时在298 nm波长下检测去甲氧基荚果蕨素，32～75 min在203 nm波长下检测重楼皂苷Ⅶ、重楼皂苷Ⅱ和重楼皂苷Ⅰ；体积流量：0.9 mL/min；进样量：20 μL。结果 去甲氧基荚果蕨素、重楼皂苷Ⅶ、重楼皂苷Ⅱ和重楼皂苷Ⅰ分别在4.14～82.80 μg/mL（r=0.9999）、3.80～76.00 μg/mL（r=0.9995）、4.94～98.80 μg/mL（r=0.9998）、7.57～151.40 μg/mL（r=0.9997）与峰面积具有较好的线性关系。平均回收率分别为99.26%、97.62%、98.27%、98.90%，RSD值分别为0.87%、1.39%、1.14%、1.15%。结论 建立的HPLC法同时测定青贯解毒颗粒中去甲氧基荚果蕨素、重楼皂苷Ⅶ、重楼皂苷Ⅱ和重楼皂苷Ⅰ，方法操作准确、简便，可作为青贯解毒颗粒的质量控制方法。

Objective To develop an HPLC method for determination of demethoxymatteucinol, chonglousaponin Ⅶ, chonglousaponin Ⅱ, and chonglousaponin Ⅰ in Qingguan Jiedu Granules. Methods The determination was carried out on Agilent Zorbax SB C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of phase of A:methanol-acetonitrile (2:1) and phase B:water with gradient elution. The detection wavelengths were 298 nm in 0-32 min (determination of demethoxymatteucinol) and 203 nm in 32-75 min (determination of chonglousaponin Ⅶ, chonglousaponin Ⅱ, and chonglousaponin Ⅰ). The flow rate was 0.9 mL/min, and volume of injection was 20 μL. Results There were good linear relationships of demethoxymatteucinol, chonglousaponin Ⅶ, chonglousaponin Ⅱ, and chonglousaponin Ⅰ in the concentration ranges of 4.14-82.80 μg/mL (r=0.9999), 3.80-76.00 μg/mL (r=0.9995), 4.94-98.80 μg/mL (r=0.9998), and 7.57-151.40 μg/mL (r=0.9997) between peak areas, respectively. The average recoveries were 99.26%, 97.62%, 98.27%, and 98.90% with RSD 0.87%, 1.39%, 1.14%, and 1.15%, respectively. Conclusion The established method can been successfully used for simultaneous determination of demethoxymatteucinol, chonglousaponin Ⅶ, chonglousaponin Ⅱ, and chonglousaponin Ⅰin Qingguan Jiedu Granules, and the method is accurate and simple which can be used in quantity control for Qingguan Jiedu Granules.