目的 观察消岩汤对环磷酰胺诱导Lewis肺癌小鼠造血微环境的影响,探究消岩汤对Lewis肺癌小鼠骨髓抑制的造血微环境的改善作用及其作用机制。方法 SPF级Lewis肺癌小鼠随机分为对照组、模型组、重组人粒细胞刺激因子组和消岩汤组,每组各10只。对照组和模型组ig 0.02 mL/g生理盐水。人粒细胞刺激因子组在造模后sc重组人粒细胞刺激因子注射液15 ng/g,1次/d,连续给药7 d。消岩汤组在造模后ig 2.9 g/mL消岩汤,2次/d,0.2 mL/次,连续给药7 d。采用流式细胞仪分析细胞周期,通过细胞培养技术检测基质细胞分化能力,采用QRT-PCR法检测骨髓细胞Bcl-2和Bax mRNA的基因表达。结果 与模型组比较,消岩汤能促进骨髓细胞从G₀/G₁期向S、G₂/M期转化(P<0.05);能一定程度上缓解化疗导致的成纤维细胞集落生成单位(CFU-F)形成减少(P<0.05);能上调抗凋亡基因Bcl-2 mRNA的表达((P<0.05)。结论 消岩汤能够促进骨髓细胞向增殖周期转化,上调Bcl-2 mRNA表达,改善造血微环境,从而缓解Lewis肺癌骨髓抑制对小鼠造血功能的影响。

Objective To observe the effects of Xiaoyan Decoction on hemopoietic microenvironment in Lewis lung cancer mice with myelosuppression induced by cyclophosphamide, and discuss the improvement on hemopoietic microenvironment in Lewis lung cancer mice with myelosuppression and to explore its mechanism. Methods SPF Lewis lung cancer mice were randomly divided into control, model, recombinant human granulocyte colony-stimulating factor (rhG-CSF), and Xiaoyan Decoction groups, and each group had 10 mice. Mice in the control and model groups were ig normal saline 0.02 mL/g. Mice in the rhG-CSF group were sc Recombinant Human Granulocyte Colony-Stimulating Factor Injection 15 ng/g after be established myelosuppression models, once daily, and be treated for 7 d. Mice in the Xiaoyan Decoction group were ig Xiaoyan Decoction 2.9 g/mL afterbe established myelosuppression models, twice daily, 0.2 mL/time, and be treated for 7 d. The cell cycles were analyzed by flow cytometry, differentiation of stromal cell were determined by cell culture technique, and express levels on Bcl-2 mRNA and Bax mRNA of bone marrow cells were checked by qRT-PCR method. Results Compared with the model group, Xiaoyan decoction could promote the bone marrow cells from G₀/G₁ phase to S and G₂ /M phase transformation in the cell cycle (P < 0.05), could inhibit the reduction of fibroblast colony forming unit (CFU-F) formation induced by chemotherapy to a certain extent(P < 0.05), and could up-regulate the expression of anti apoptotic gene Bcl-2 mRNA (P < 0.05). Conclusion Xiaoyan Decoction can promote the transformation of bone marrow cells to proliferation cycles, up-regulate the expression of Bcl-2 mRNA gene, improve hemopoietic microenvironment, and relieve the effect of hematopoietic function in Lewis lung cancer mice with myelosuppression.

国家自然科学基金资助项目(81273937);天津市应用基础与前沿技术研究计划项目(13JCQNJC10900);天津市科技计划项目(12ZCDZSY15800)