目的 建立不同来源的千金藤植物中千金藤素的HPLC测定方法。方法 采用AngelaC₁₈色谱柱(250mm×4.6mm,5μm);流动相:乙腈–水(52:48,含0.5%三乙胺、0.01%磷酸);检测波长:282nm;柱温:30℃;体积流量:1.0mL/min;进样体积:20μL。结果 千金藤素质量浓度在9~216μg/mL时与峰面积值呈良好的线性关系(r＝0.9997),平均回收率为96.0%,RSD值为2.0%(n＝6)。产自湖南的千金藤(No.13)、产自广西的白药子(No.17)中千金藤素的量较高,分别为0.0139%、0.1221%;同一植株的不同用药部位(No.12~15)中千金藤素的量也不尽相同,湖南产千金藤中千金藤素主要分布在茎中,其质量分数为0.0139%。结论 该方法操作简便、准确、重复性好,可用于千金藤植物千金藤、地不容和白药子中千金藤素的测定。

Objective To establish an HPLC method for determination of cepharanthine in the plants of StephaniaLour. from various habitats and parts. Methods The determination was carried out on Angela C₁₈ column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water (52:48, 0.5% triethylamine and 0.01% phosphoric acid). The column temperature was set at 30 ℃ at a flow rate of 1.0 mL/min. The injection volume was 20 μL. Results Cepharanthine had a good linearity in the ranges of 9-216 μg/mL (r = 0.999 7). The average recovery rate was 96.0% with RSD value at 2.0%. Contents of cepharanthine in Stephania japonica sample (No.13) from Hunan province and S.cepharantha sample (No.17) from Guangxi province were the highest. Contents of cepharanthine in various plant parts of samples (No.12-15) were different, and that in S. japonica from Hunan province were generally found in the stem, which content reached to 0.013 9%. Conclusion The established method is simple, sensitive, and highly reproducible, which is suitable for the determination of cepharantine in S. japonica, S. epigaea, and S.cepharantha.

国家自然科学基金资助项目(U1403102,81473108);辽宁省自然科学基金资助项目(2015020732)