目的 探讨补骨脂素对H₂O₂诱导PC-12细胞氧化损伤的保护作用,并考察其作用机制。方法 PC-12细胞分为对照组、模型组、Tempol组和补骨脂素组。对照组中未加入补骨脂素和H₂O₂;模型组中加入400μmol/LH₂O₂;Tempol组中加入100μmol/LTempol及400μmol/LH₂O₂;补骨脂素组中加入400μmol/LH₂O₂及1、5、10、20μmol/L补骨脂素。CCK-8法测定PC-12细胞的存活率,考察对PC-12细胞形态的影响,PI/Annexin-V、Hoechst染色检测细胞凋亡率,蛋白质印迹法检测凋亡蛋白表达。结果 5、10、20μmol/L补骨脂素均可以显著提高PC-12细胞内的吸光度值(P<0.01),细胞存活率提高较明显,与模型组比较差异具有统计学意义(P<0.05)。补骨脂素1、5、10、20μmol/L组凋亡率明显低于模型组(P<0.05),并且随着补骨脂素浓度的增加,PC-12细胞的凋亡率逐渐下降。随着补骨脂素作用时间的增加,各组PC-12细胞凋亡率的增加幅度相近,但明显低于模型组(P<0.05)。随着补骨脂素浓度的增加,对磷酸化Bad、Bcl-XL水平的上调作用越明显。结论 补骨脂素对H₂O₂诱导PC-12细胞损伤具有保护作用,其作用机制是通过上调磷酸化Bad和Bcl-XL表达来抑制细胞凋亡。

Objective To discuss the protective effect of psoralen on PC-12 cell damage induced by H₂O₂, and investigate its mechanism. Methods PC-12 cells were divided into control model, Tempol, and psoralen groups. Cells in the control group did not receive damage or treatments, the model group was established by 400 μmol/L H₂O₂-damage, Tempol group was cultured by 100 μmol/L Tempol and 400 μmol/L H₂O₂, and psoralen groups were cultured by 400 μmol/L H₂O₂ and 1, 5, 10, and 20 μmol/L psoralen. Cell survival rates in four groups were detected by CCK-8 method, apoptosis rates were detected by PI/Annexin and Hoechst methods, and protein expression were detected by Western blotting. Results Compared with the model group, psoralen (1, 5, 10, and 20 μmol/L) could significantly improve absorbance values (P < 0.01) and cell survival rates (P < 0.05) of PC-12 cells. Apoptosis rates of psoralen (1, 5, 10, and 20 μmol/L) groups were significantly lower than those of model group (P < 0.05). And with increase of psoralen concentration, apoptosis rates of PC-12 cells were gradually decreased. With longer action time, the increase amplitudes of PC-12 cells apoptosis rates among groups were similar, and obviously lower than those of model group (P < 0.05). Psoralen could significantly up-regulate levels of p-Bad and Bcl-XL with increase of concentrations. Conclusion Psoralen has protective effect on PC-12 cell damage induced by H₂O₂, which is related to up-regulation of p-Bad and Bcl-XL to inhibit the apoptosis of PC-12 cell.