目的 研究元胡止痛滴丸对大鼠实验性偏头痛的镇痛作用及其作用机制。方法 Wistar大鼠随机分为对照组、模型组、正天丸(3.0 g/kg)组以及元胡止痛滴丸0.4、0.8、1.6 g生药/kg组,每组10只,ig给药,给药体积0.5 mL/100 g,1次/d,连续给药3 d。对照组和模型组给予等体积的去离子水。末次给药后30 min,除对照组外,其余各组大鼠sc硝酸甘油注射液10 mg/kg,复制大鼠实验性偏头痛模型。立即记录大鼠搔头潜伏期以及60 min内的搔头次数,计算搔头潜伏期延长率和搔头次数抑制率。测定大鼠脑组织和血清中多巴胺(DA)、5-羟色胺(5-HT)、β-内啡肽(β-EP)、降钙素基因相关肽(CGRP)、NO水平。结果 与模型组比较,元胡止痛滴丸0.8、1.6 g生药/kg组偏头痛大鼠的搔头次数显著减少(P<0.05、0.01),并呈剂量相关性。与模型组比较,元胡止痛滴丸0.8 g生药/kg组DA、NO水平显著降低(P<0.05),1.6 g生药/kg组5-HT、DA、NO水平显著降低(P<0.01、0.001),β-EP水平显著升高(P<0.05),但对CGRP水平基本无影响。结论 元胡止痛滴丸在0.8~1.6 g生药/kg对大鼠实验性偏头痛具有显著的镇痛作用,其作用机制可能与调节5-HT、NO、DA、β-EP等与疼痛相关的血管活性物质及神经递质的水平有关。

Objective To study analgesic effect of Yuanhu Zhitong Dropping Pills on experimental migraine in rats induced by nitroglycerin and explore its mechanism.Methods Wistar rats were randomly divided into control group, model group, Zhengtian Pills (3.0 g/kg) group, and Yuanhu Zhitong Dropping Pills (0.4, 0.8, and 1.6 crude drug g/kg) groups, and each group had 10 rats. Rats were ig administered with corresponding drugs 0.5 mL/100 g except the control group and model group, once daily, and treated for 3 d. Rats except in the control group were sc administered with Nitroglycerin Injection 10 mg/kg to make migraine model after last administration 30 min. The latent periods and frequencies in 60 min of scratching head reaction were observed, and the prolonged rate of latent period and inhibitory rate of scratching head were calculated. The levels of DA, 5-HT, β-EP, CGRP, and NO in brain tissues and serum were determined.Results Compared with the model group, the frequency of scratching head of rats in Yuanhu Zhitong Dropping Pills (0.8 and 1.6 crude drug g/kg) groups were significantly reduced (P< 0.05, 0.01), and showed dose dependent. Compared with the model group, levels of DA and NO in Yuanhu Zhitong Dropping Pills (0.8 crude drug g/kg) group were markedly decreased (P< 0.05). The levels of DA and NO in Yuanhu Zhitong Dropping Pills (1.6 crude drug g/kg) group were significantly decreased (P< 0.01, 0.001), but the level of β-EP was significantly increased (P< 0.05), and there was no difference on the level of CGRP between the model group and Yuanhu Zhitong Dropping Pills (1.6 crude drug g/kg) group.Conclusion Yuanhu Zhitong Dropping Pills has significantly analgesic effect on experimental migraine model of rats, which may be related to the levels of vasoactive substances and neurotransmitters related to pain, such as 5-HT, NO, DA, and β-EP.