目的 建立HPLC-ELSD法同时测定白果药材中银杏内酯A、银杏内酯B和银杏内酯C 3个萜杏内酯成分。方法 采用Agilent 5TC C₁₈色谱柱(250 mm×4.6 mm,5 μm),以甲醇-四氢呋喃-水(25:10:65)为流动相,体积流量1 mL/min,柱温30 ℃,蒸发光散射检测器检测,对照品进样量为10、20 μL,供试品溶液进样量为5~20 μL。结果 银杏内酯A在0.37~5.92 μg、银杏内酯B在2.8~44.8 μg、银杏内酯C在0.65~10.4 μg时线性关系良好。银杏内酯A、银杏内酯B、银杏内酯C平均回收率分别为92.40%、95.03%、91.29%,RSD值分别为2.75%、2.06%、2.88%。结论 本法操作简单,重复性好,为白果药材的质量控制提供了依据。

Objective To establish an HPLC-ELSD method for the determination of three terpene lactones ginkgolide A, ginkgolide B, and ginkgolide C in Ginkgo Semen. Methods The determination was carried out on Agilent 5TC C₁₈ column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-tetrahydrofuran-water (25:10:65). The column temperature was set at 30 ℃ at a flow rate of 1.0 mL/min. ELSD was used as detector to determine the terpene lactones. The injection volumes were 10 and 20 μL for reference substance solution, and 5—20 μL for sample solution. Results The linear ranges of ginkgolide A, ginkgolide B, and ginkgolide C were 0.37—5.92 μg, 2.8—44.8 μg, and 0.65—10.4 μg, respectively. The average recoveries of three components were 92.40%, 95.03%, and 91.29% with RSD 2.75%, 2.06%, and 2.88%, respectively. Conclusion The method is simple and repeatable, which can be used in quantity control for Ginkgo Semen.

国家重大新药创制科技重大专项(2013ZX09402203)