目的 建立HPLC同时测定八珍液中的地黄苷A、地黄苷D、去氢土莫酸和茯苓酸的方法。方法 Kromasil C₁₈色谱柱(200 mm×4.6 mm,5 μm);流动相：乙腈–0.05%磷酸溶液,梯度洗脱;检测波长：203 nm(0～34 min,检测地黄苷A和地黄苷D)、210 nm(34～65 min,检测去氢土莫酸和茯苓酸);体积流量1.0 mL/min。结果 地黄苷A、地黄苷D、去氢土莫酸和茯苓酸分别在5.76～115.20、4.05～81.00、6.10～122.00、6.35～127.00 μg/mL线性关系良好;平均回收率分别为98.90%、99.08%、98.71%、97.95%,RSD值分别为1.49%、1.19%、1.63%、0.92%。结论 该方法操作简便,准确,重复性好,可作为八珍液的质量控制方法。

Objective To establish an HPLC method for simultaneous determination of rehmaionoside A, rehmaionoside D, dehydrotumulosic acid, and pachymic acid in Bazhen Solution. Methods The determination was carried out on Kromasil C₁₈ column (200 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile - 0.05% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL/min. The column temperature was set at 30 ℃, and the detection wavelengths were 203 nm in 0 — 34 min (determination of rehmaionoside A and rehmaionoside D) and 210 nm in 34 — 65 min (determination of dehydrotumulosic acid and pachymic acid). Results There were good linear relationships of rehmaionoside A, rehmaionoside D, dehydrotumulosic acid, and pachymic acid in the concentration ranges of 5.76 — 115.20 μg/mL, 4.05 — 81.00 μg/mL, 6.10 — 122.00 μg/mL, and 6.35 — 127.00 μg/mL. The average recoveries were 98.90%, 99.08%, 98.71%, and 97.95% with RSD 1.49%, 1.19%, 1.63%, and 0.92%, respectively. Conclusion The method is simple, accurate, and repeated, which can be used in quantity control for Bazhen Solution.