目的 研究芦丁对流感病毒的体外抑制作用并探讨其机制。方法 利用流感病毒感染MDCK细胞模型,观察芦丁对流感病毒的体外抑制作用;结合流感病毒感染前、感染同时及感染后加药的不同方式探索芦丁作用于流感病毒生命周期的具体阶段;采用荧光底物法考察芦丁对流感病毒神经氨酸酶的抑制作用。结果 芦丁对A/PuertoRico/8/1934(H1N1)、A/FM1/1/47(H1N1)、A/Human/Hubei/3/2005(H3N2)、A/Beijing/32/92 (H3N2)4株流感病毒均具有体外抑制作用,其中对A/PuertoRico/8/1934(H1N1)株的半数有效浓度(EC₅₀)最小,选择指数(SI)最大,体外药效最好。芦丁在流感病毒感染后给药的效果最为明显,对流感病毒A/FM1/1/47(H1N1)的IC₅₀最小,对神经氨酸酶活性抑制作用相对最好。结论 芦丁抑制了流感病毒神经氨酸酶活性,具有较好的体外抗流感病毒作用。

Objective To study the inhibition of rutin against influenza virus in vitro and explore its mechanism. Methods To establish the MDCK cell models infected by influenza virus, and inhibition of rutin against influenza virus in vitro was observed. The specific stages of influenza virus infection treated by rutin were evaluated before, during and after infection. Inhibition of rutin against neuraminidase activities of influenza virus were studied by fluorescent substrate method. Results Rutin exhibited antiviral activity against A/PuertoRico/8/1934(H1N1), A/FM1/1/47(H1N1), A/Human/Hubei/3/2005(H3N2), and A/Beijing/32/92(H3N2), especially on A/PuertoRico/8/1934(H1N1) with minimum EC₅₀, maximum SI, and best efficacy in vitro. The effect is the most obvious treated by rutin administration after infected with influenza virus. With IC₅₀ value on A/FM1/1/47(H1N1) influenza virus minimum, rutin inhibited the relatively the best activity of neuraminidase. Conclusion Rutin inhibits activity of neuraminidase of influenza virus, and has good anti-influenza effects in vitro.

国家科技部重大新药创制项目(2013ZX09402203)