目的 建立HPLC-DAD法同时测定欧亚旋覆花中绿原酸、1,5-二咖啡酰奎宁酸、芦丁、菠叶素、槲皮素、木犀草素、6-甲氧基木犀草素。方法 Diamonsil C₁₈色谱柱(200 mm×4. 6 mm,5 μm);流动相为乙腈-0.4%枸橼酸水溶液,梯度洗脱;体积流量1 mL/min;柱温30 ℃;检测波长360 nm;进样量:10 μL。结果 绿原酸、1,5-二咖啡酰奎宁酸、芦丁、菠叶素、槲皮素、木犀草素、6-甲氧基木犀草素分别在0.118～2.360、0.261～5.220、0.030～0.600、0.052～0.140、0.081～0.162、0.041～0.820、0.030～0.600 μg与峰面积呈良好的线性关系。平均回收率分别为97.22%、97.43%、97.52%、99.48%、98.87%、98.76%、98.39%,RSD值分别为0.92%、1.16%、1.50%、1.56%、0.79%、0.76%、1.62%(n＝6)。结论 该方法准确、简便、重复性好,可用于欧亚旋覆花药材中成分的质量控制。

Objective To develop an HPLC-DAD method for simultaneous determination of chlorogenic acid, 1,5-dicaffeoylquinic acid, rutin, spinacetin, quercetin, luteolin, and 6-methoxyluteolin in Inula britannica from various habitats. Methods The analysis was performed on Diamonsil C₁₈ column (250 mm × 4.6 mm, 5 μm) with 0.4% citric acid-acetonitrile as mobile phases for gradient elution. The flow rate was 1.0 mL/min. The detection wavelength was set at 360 nm. The column temperature was 30 ℃ with injection volume of 10 μL. Results The calibration curves were linear in the ranges of 0.118-2.360, 0.261-5.220, 0.030-0.600, 0.052-0.140, 0.081-0.162, 0.041-0.820, and 0.030-0.600 μg for chlorogenic acid, 1,5-dicaffeoylquinic acid, rutin, spinacetin, quercetin, luteolin, and 6-methoxyluteolin, respectively. The average recoveries were 97.22%, 97.43%, 97.52%, 99.48%, 98.87%, 98.76%, and 98.39% with RSD values of 0. 92%, 1.16%, 1.50%, 1.56%, 0.79%, 0.76%, and 1.62% (n = 6), respectively. Conclusion The method is accurate, convenient and reproducible for quality control for multicomponent in I. britannica.