目的 建立HPLC法同时测定三才封髓丸中地黄苷A、地黄苷D、黄柏碱和木兰花碱的方法。方法 采用Phenomenex RP C₁₈色谱柱(250 mm×4.6 mm,5 μm);流动相为乙腈-0.1%磷酸溶液,梯度洗脱;0～28 min地黄苷A和地黄苷D检测波长为203 nm,28～60 min黄柏碱和木兰花碱的检测波长为225 nm;体积流量为1.0 mL/min;柱温为30 ℃;进样量20 μL。结果 地黄苷A、地黄苷D、黄柏碱和木兰花碱在4.52～90.40、4.10～82.00、5.35～107.00、5.28～105.60 μg/mL线性关系良好;平均回收率分别为98.86%、96.95%、99.15%、96.98%,RSD值分别为1.38%、1.24%、1.28%、0.85%。结论 建立的方法简便、准确、灵敏度高、重复性好,可用于三才封髓丸的质量控制。

Objective To establish an HPLC method for simultaneous determination of rehmannioside A, rehmannioside D, phellodendrine, and magnoflorine in Sancai Fengsui Pills. Methods The HPLC method was carried out on a Phenomenex RP C₁₈ column (250 mm × 4.6 mm, 5 μm) with a mobile phase consisted of acetonitrile-0.1% phosphoric acid solution for gradient elution. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ with injection volume of 20 μL. The detection wavelength for rehmannioside A and rehmannioside D was set at 203 nm, and that for phellodendrine and magnoflorine was set at 225 nm. Results Rehmannioside A, rehmannioside D, phellodendrine, and magnoflorine had good linearity in the ranges of 4.52-90.40 μg/mL, 4.10-82.00 μg/mL, 5.35-107.00 μg/mL, and 5.28-105.60 μg/mL, respectively. And the average recoveries were 98.86%, 96.95%, 99.15%, and 96.98% with RSD values of 1.38%, 1.24%, 1.28%, and 0.85%, respectively. Conclusion The method is simple, accurate, and sensitive, with good repeatability, which can be used to evaluate the quality of Sancai Fengsui Pills.