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[摘要]
目的 探讨宁泌泰胶囊的抗炎作用及其作用机制。方法 采用1%角叉菜胶致小鼠足趾肿胀、2%巴豆油丙酮溶液致小鼠耳肿胀模型,观察宁泌泰胶囊(0.5、1.5、4.5 g/kg)对小鼠肿胀的抑制作用。考察25、50、100 μg/mL宁泌泰胶囊加入RAW264.7细胞作用48、72 h后的细胞毒性。运用双荧光素酶报告基因系统检测宁泌泰胶囊对NF-κB转录活性的影响。结果 与模型组比较,宁泌泰胶囊(4.5 g/kg)对角叉菜胶致小鼠足肿胀、巴豆油致小鼠耳肿胀具有明显的抑制作用(P< 0.05)。宁泌泰胶囊对RAW264.7细胞没有细胞毒性。50、100 μg/mL宁泌泰胶囊内容物对NF-κB的转录激活具有明显的抑制作用(P< 0.01)。结论 宁泌泰胶囊具有抗炎作用,其作用机制可能是通过NF-κB通路来抑制炎症过程。
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[Abstract]
Objective To study the anti-inflammatory effect of Ningmitai Capsules and its mechanism. Methods The models of 1% carrageenan-induced mice paw edema and 2% oleum crotonis-induced ear edema were established, and the inhibition of swelling in mice adminisered with Ningmitai Capsules were observed. Cytotoxicity of 25, 50, and 100 μg/mL Ningmitai Capsules on RAW264.7 cells were studied after 48 and 72 h. Dual luciferase report gene system was used to detect the transcriptional activity of NF-κB. Results Compared with model group, Ningmitai Capsules with the dosage of 4.5 g/kg could obviously inhibit the mice foot swelling induced by carrageenan and the ear edema induced by oleum crotonis (P < 0.05). There was no cytotoxicity of Ningmitai Capsules on RAW264.7 cells. Ningmitai Capsules with the concentration of 50 and 100 μg/mL could inhibit the transcriptional activity of NF-κB (P < 0.01). Conclusion Ningmitai Capsules have anti-inflammatory effect, which is related to inhibition of inflammatory processes through NF-κB pathway.
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