目的 探讨金黄凝胶对大鼠急性软组织损伤的治疗作用.方法 随机选取10 只大鼠作为对照组,将其余大鼠随机分为模型组、双氯芬酸钠凝胶(0.010 g)组、青鹏软膏(0.300 g)组、金黄散膏(0.069 g)组以及金黄凝胶0.033、0.069、0.138 g 组.采用砝码砸伤法造成大鼠急性软组织损伤.造模后24 h 开始给药,各组分别以各受试药物均匀涂抹大鼠左后肢砸伤部位.观察砸伤部位肿胀程度,计算肿胀度;测定造模部位痛阈值;记录损伤指数;测定血清白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、前列腺素E₂(PGE₂)、一氧化氮(NO)的含量;观察造模部位的病理变化.结果 与模型组比较,给药第3、5 天金黄凝胶0.033、0.069、0.138 g 组肿胀度均显著降低、痛阈值显著升高(P< 0.05、0.01);金黄凝胶0.033、0.069、0.138 g 组给药5 d 后损伤指数显著降低(P< 0.05、0.01).与模型组比较,金黄凝胶0.033、0.069、0.138 g 组PGE₂显著降低(P< 0.01),金黄凝胶0.138 g 组IL-6、0.069 g 组NO 显著降低(P< 0.05).各给药组大鼠对病变均有不同程度改善,除金黄凝胶0.033 g 组外,各给药组大鼠肌肉病变均显著降低(P< 0.05、0.01).结论 金黄凝胶对急性软组织损伤具有治疗作用.

Objective To explore the therapeutic effects of Jinghuang Gel on acute soft tissue injury in rats. Methods Except 10 rats were selected as control group, the other rats were randomly divided into model group, Diclofenac Sodium Gel (0.010 g) group, Qingpeng Ointment (0.300 g) group, and Jinghuang Gel (0.033, 0.069, and 0.138 g) groups. Acute soft tissue injury models in rats were caused by crushing method. After 24 h, rats were smeared with different medicines on left hind limb injured site. The degree of swelling in injured site was observed, and swelling degree was calculated. Pain threshold was determined. Damage index was recorded. Levels of IL-1β, IL-6, PGE₂, and NO in serum were determined. And pathological changes were detected in injured site before and after the administration. Results Compared with the model group, swelling degrees of Jinghuang Gel (0.033, 0.069, and 0.138 g) groups were significantly decreased, and pain thresholds were obviously increased (P < 0.05, 0.01) after administration for 3 and 5 d; The damage index of those groups was significantly decreased (P < 0.05, 0.01) after administration for 5 d. Compared with the model group, levels of PGE₂ of Jinghuang Gel (0.033, 0.069, and 0.138 g) groups were significantly decreased (P < 0.05, 0.01), and levels of IL-6 in Jinghuang Gel 0.138 and 0.069 g groups were also significantly decreased (P < 0.05). The injury degree of rats administered was improved, and muscle injury scores were significantly decreased (P < 0.05, 0.01) except Jinghuang Gel 0.033 g group. Conclusion Jinghuang Gel has therapeutic effect on acute soft tissue injury.

国家重大新药创制项目(2013ZX09402203)