目的 明确麸炒苍术健脾的活性部位.方法 选用番泻叶水煎液ig 制备大鼠脾虚模型.考察麸炒苍术挥发油、二氯甲烷萃取物、正丁醇萃取物和多糖对脾虚大鼠体质量、血清胃泌素、血管活性肠肽、血清淀粉酶和D-木糖水平的影响.结果 与模型组比较,除多糖组外其余各给药组在治疗后体质量均明显增加(P< 0.05);大鼠血清淀粉酶、D-木糖、血清胃泌素水平显著增加,血管活性肠肽水平降低,差异均具有显著性(P< 0.05).结论 除多糖无健脾作用外,麸炒苍术其余各部位健脾作用明显,并且健脾的活性部位主要为低极性成分.

Objective To confirm active parts from Atractylodis Rhizoma stir-baked with bran on invigorating the spleen. Methods Spleen insufficiency model was made by ig administration with extract of Sennae Folium. Body weight and levels of gastrin, vasoactive intestinal peptide, serum amylase, and D-xylose in the serum of rats were studied after ig administration with volatile oil, CH₂Cl₂ active parts, n-BuOH active parts, and polysaccharde. Results Compared with model group, the body weights in the volatile oil group, CH₂Cl₂ active parts, and n-BuOH active parts obviously increased (P < 0.05). And the levels of gastrin, serum amylase, and D-xylose were increased, but the levels of vasoactive intestinal peptide significantly decreased (P < 0.05). Conclusion Active parts except polysaccharide from Atractylodis Rhizoma stir-baked with bran show the functions of vigorating the spleen, and low polarity components are active.

国家自然科学基金资助项目(81202919);国家中医药管理局中医药行业科研专项项目(20110700712)