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[摘要]
目的 建立多波长HPLC梯度洗脱法同时测定更年乐片中朝藿定C、淫羊藿苷、川续断皂苷Ⅵ和大花双参苷A的方法.方法 依利特C18柱(250 mm×4.6 mm,5 μm);流动相为乙腈–0.1%磷酸溶液,梯度洗脱;检测波长:270 nm(朝藿定C和淫羊藿苷)、210 nm(川续断皂苷Ⅵ)、230 nm(大花双参苷A);体积流量为1.2 mL/min;柱温为室温;进样量20 μL.结果 朝藿定C、淫羊藿苷、川续断皂苷Ⅵ和大花双参苷A分别在7.14~142.80 μg/mL(r=0.999 8)、5.64~112.80 μg/mL(r=0.999 6)、6.35~127.00 μg/mL(r=0.999 5)、7.90~158.00 μg/mL(r=0.999 3)与其峰面积呈良好的线性关系;朝藿定C、淫羊藿苷、川续断皂苷Ⅵ和大花双参苷A的平均回收率分别为99.24%、96.93%、97.81%、98.32%,RSD值分别为1.28%、0.94%、1.24%、1.50%.结论 该方法是一种快速、灵敏、准确的分析方法,可作为更年乐片的质量控制方法.
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[Abstract]
Objective To develop an HPLC method for the simultaneous determination of epimedin C, icariin, asperosaponin Ⅵ, and triplostoside A in Gengnianle Tablets. Methods The analysis was performed on Elite C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile-0.1% phosphoric acid solution as mobile phases at the flow rate of 1.2 mL/min for gradient elution. Detection with variable wavelength was used, and set at 270 nm for epimedin C and icariin, 210 nm for asperosaponin Ⅵ, and 230 nm for triplostoside A. The column temperature was room temperature with injection volume of 20 μL. Results Epimedin C, icariin, asperosaponin Ⅵ, and triplostoside A had good linearity in the ranges of 7.14—142.80 μg/mL (r = 0.999 8), 5.64—112.80 μg/mL (r = 0.999 6), 6.35—127.00 μg/mL (r = 0.999 5), and 7.90—158.00 μg/mL (r = 0.999 3), respectively. The average recoveries were 99.24%, 96.93%, 97.81%, and 98.32% with RSD 1.28%, 0.94%, 1.24%, and 1.50%, respectively. Conclusion The method is simple, sensitive, and accurate, which can be used in quantity control for Gengnianle Tablets.
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