目的 考察毛叶疏花蔷薇果提取物的亚慢性毒性.方法 选用96只健康SD大鼠随机分为4组,雌雄各半,分别ig给予0、1.25、2.50、5.00 g/kg毛叶疏花蔷薇果提取物,连续90 d,观察动物生长情况.试验中、末期对动物的血液学和血清生化指标进行检测,试验末计算动物主要脏器的脏器系数,并进行组织病理学检查.结果 试验期间动物无死亡.试验末各剂量组动物体质量增长值、进食量、食物利用率、脏器质量、脏器系数与对照组比较差异均无统计学意义.在试验中、末期各剂量组动物血液学检测指标与对照组比较差异无统计学意义.血清生化检测发现,试验中期5.00 g/kg剂量组雌雄大鼠血清中的肌酐(CR)和雌性大鼠血清中的尿素氮(BUN)水平显著升高;末期5.00 g/kg剂量组雄性大鼠血清中的CR水平仍居高不下,与对照组比较差异均有统计学意义(P< 0.05、0.01),而其他指标均在正常值范围内.病理组织学检查发现5.00 g/kg剂量组部分动物肾小管和小肠可见相关病理改变.结论 在本实验条件下,5.00 g/kg毛叶疏花蔷薇果提取物对SD大鼠的肾和小肠有损伤作用,最大无作用剂量为2.50 g/kg.

Objective To study the subchronic toxicity of Rosa laxa var. mollis fruit extract. Methods SD rats (96) were divided randomly into four groups, and each group consisted of 12 males and 12 females. The animals in four groups were ig administered with R. laxa var. mollis fruit extract at doses of 0 (control), 1.25, 2.50, 5.00 g/kg for 90 d, respectively. During the entire course of the study, the rats were observed daily. The hematological and serum biochemical examinations were conducted after 16 h of starvation at 45th day as an intermittent study and 90th day as a terminal. All animals were calculated organ coefficients and studied for histological changes. Results At the end of the study, no deaths were observed in all groups. There were no differences in body weight gains, food intakes, food availability, and organ coefficients compared to the control (P> 0.05). For the hematological analyses, no parameters demonstrated significant changes in all groups during the entire study. During the serum biochemical analyses, there were significant increases of creatinine (CR) level in 5.00 g/kg both sexes and blood urea nitrogen (BUN) level in 5.00 g/kg female rats at the 45th day. Thereafter, significant increase of CR level in 5.00 g/kg male rats were observed at the end of the study (P <0.05, 0.01), but the other indexes were in the normal range. In 5.00 g/kg dose group, remarkable histopathological changes were observed in kidney and intestine of the SD rats. Conclusion R. laxa var. mollis fruit extract could be harmful to kidneys and intestines of the SD rats at 5.00 g/kg dose. No observed adverse effect level (NOAEL) is 2.50 g/kg.

湖南省中医药科研计划项目(2013106)