目的 探讨桂枝茯苓胶囊对子宫肌瘤大鼠的治疗作用及其机制.方法 采用注射苯甲酸雌二醇和黄体酮复制大鼠子宫肌瘤模型,造模大鼠按体质量随机分为模型组、他莫昔芬(枸橼酸他莫昔芬片3.6 mg/kg)组以及桂枝茯苓胶囊0.14、0.27、0.54 g/kg组,另设对照组.连续给药8周后处死大鼠,取子宫、脾脏、胸腺并计算其脏器系数,比较子宫病理评分和组织形态,放射免疫分析方法检测血清中雌二醇(E2)、孕酮(P)及子宫组织中表皮生长因子(EGF)水平.结果 桂枝茯苓胶囊显著降低大鼠子宫系数,减轻子宫壁平滑肌增生,显著降低血清E2和子宫组织EGF水平.结论 桂枝茯苓胶囊对大鼠子宫肌瘤具有治疗作用,其作用机制可能与降低血清E2、子宫组织EGF水平有关.

Objective To discuss the therapeutical effect of Guizhi Fuling Capsule on uterine leiomyoma in rats and its mechanism. Methods Uterine leiomyoma model in rats was made by injection estradiol benzoate and progesterone. The rats with uterine leiomyoma were randomly divided into model group, tamoxifen (Tamoxifen Citrate Tablets 3.6 mg/kg) group, and Guizhi Fuling Capsule (0.14, 0.27, and 0.54 g/kg) groups. There was also a control group. After treatment for eight weeks, the rats were killed by cervical dislocation. The organ coefficients of the uterus, thymus, and spleen were calculated. Pathological score and pathological changes of uterus tissue were detected. The levels of estradiol (E2), progesterone (P) in serum and epidermal growth factor (EGF) in uterus tissue serum were determined by radioimmunoassay. Results After treatment, Guizhi Fuling Capsule significantly reduced the uterus coefficient, alleviated hyperplasia of smooth muscle in uterus wall, significantly reduced the levels of E2 in serum and EGF in uterine tissue of rats. Conclusion Guizhi Fuling Capsule has therapeutical effect on uterine leiomyoma in rat, and the mechanism may be associated with reduction of E2 levels in serum and EGF levels in uterine tissue.

国家科技重大专项课题(2013ZX09402203)