目的 建立测定脑血栓片中苦杏仁苷、羟基红花黄色素A、芍药苷、阿魏酸、丹酚酸B和丹参酮Ⅱ_A的HPLC-DAD法。方法 采用HPLC-DAD法,Agilent Poroshell 120 SB-C₁₈色谱柱(100 mm×4.6 mm,2.7 μm);流动相:甲醇-0.2%磷酸溶液,梯度洗脱;检测波长分别为210 nm(苦杏仁苷)、403 nm(羟基红花黄色素A)、230 nm(芍药苷)、321 nm(阿魏酸)、286 nm(丹酚酸B)、270 nm(丹参酮Ⅱ_A);体积流量:0.7 mL/min;柱温:30 ℃;进样量3～5 μL。结果 苦杏仁苷、羟基红花黄色素A、芍药苷、阿魏酸、丹酚酸B和丹参酮Ⅱ_A 6个成分的线性范围分别为11.90～1 158.90、9.14～91.39、11.70～1 173.50、4.04～1 011.00、3.97～992.20、4.40～551.00 ng;平均回收率分别为96.47%、96.92%、99.96%、97.20%、97.57%、96.50%,RSD值分别为1.3%、1.6%、1.3%、1.7%、1.9%、0.7%。结论 所建立的方法可同时测定脑血栓片中苦杏仁苷、羟基红花黄色素A、芍药苷、阿魏酸、丹酚酸B和丹参酮Ⅱ_A。

Objective To develop an HPLC-DAD method for determination of amygdalin, hydroxysafflor yellow A, paeoniflorin, ferulic acid, salvianolic acid B, and tanshinoneⅡ_A in Naoxueshuan Tablets. Methods HPLC-DAD chromatography was used. The analysis was carried out on an Agilent Poroshell 120 SB-C₁₈ column (100 mm × 4.6 mm, 2.7 μm) with methanol - 0.2% phosphoric acid as mobile phases at the flow rate of 0.7 mL/min for gradient elution. Detection with variable wavelength were used, and set at 210 nm for amygdalin, 403 nm for hydroxysafflor yellow A, 230 nm for paeoniflorin, 321 nm for ferulic acid, 278 nm for salvianolic acid, and 270 nm for tanshinoneⅡ_A. The column temperature was 30 ℃ with injection volume of 3 —5 μL. Results The linear ranges of six components were 11.90 — 1158.90, 9.14 — 91.39, 11.70 — 1 173.50, 4.04 — 1 011.00, 3.97 — 992.20, 4.40 — 551.00 ng, respectively. The average recoveries were 96.47%, 96.92%, 99.96%, 97.20%, 97.57%, and 96.50% with RSD1.3%, 1.6%, 1.3%, 1.7%, 1.9%, and 0.7%, respectively. Conclusion This method can be used to simultaneously determine amygdalin, hydroxysafflor yellow A, paeoniflorin, ferulic acid, salvianolic acid B, and tanshinoneⅡ_A in Naoxueshuan Tablets.