目的 优化苦豆子多糖的脱色条件.方法 以多糖脱色率和多糖保留率为指标,考察双氧水(H2O2)对苦豆子多糖溶液的脱色效果.通过单因素试验考察H2O2脱色方法中双氧水加入量、脱色时间、脱色温度和pH值对苦豆子多糖脱色效果的影响,进一步采用正交试验确定苦豆子多糖脱色的最佳条件.结果 H2O2对苦豆子多糖最佳脱色条件为脱色温度60 ℃,调节pH 5,加入1.0%的H2O2,脱色反应3 h.在此条件下经验证,多糖脱色率和多糖保留率可分别达到29.83%、71.12%.结论 H2O2脱色具有较好的脱色效果,可为生产出高品质的苦豆子多糖提供依据.
Objective To optimize the decolorization conditions on Sophora alopecuroides polysaccharide. Methods The effects of H2O2 on decolorization of S. alopecuroides polysaccharide were studied with polysaccharide decolorization rate and polysaccharide retention rate as indexes. The amount of H2O2, bleaching time, bleaching temperature, and pH value were studied through single factor test in H2O2 bleaching method, and the further optimal conditions of S. alopecuroides polysaccharide were studied by orthogonal test. Results The optimal decoloring conditions for S. alopecuroides polysaccharide with H2O2 were as following: decolorization temperature of 60 ℃, pH value of 5, addition of 1% H2O2, and decolorization reaction for 3 h. Under these conditions, the polysaccharide decolorization rate and polysaccharide retention rate could achieve 29.83% and 71.12% after verification. Conclusion The method with H2O2 has good decolorizing effect, and can provide a scientific basis for the production of S. alopecuroides polysaccharide with high quality.