目的 构建寡肽转运蛋白(PEPT1)高表达的Caco-2细胞系,并用于α-常春藤皂苷缬氨酸酯吸收机制的研究.方法 0.2 nmol/L瘦素诱导Caco-2细胞7 d以增加细胞上PEPT1的表达量.通过在瘦素诱导Caco-2细胞上进行吸收抑制实验,研究α-常春藤皂苷缬氨酸酯的吸收机制.结果 相对于未经诱导的Caco-2细胞,α-常春藤皂苷缬氨酸酯在诱导的Caco-2细胞上摄取量提高了5.33倍,提示PEPT1的表达量增加了.结论 α-常春藤皂苷缬氨酸酯的吸收是由PEPT1介导的.
Objective To establish a Caco-2 system with highly-expressed peptide transporter (PEPT1), and to apply this system to the absorption of L-valyl-α-hederin. Methods Caco-2 cells were induced with 0.2 nmol/L leptin for 7 d to increase the PEPT1 expression. The uptake mechanism of L-valyl-α-hederin was studied by inhibition absorption in leptin-treated Caco-2 cells. Results The uptake of L-valyl-α-hederin was 5.33 times higher in leptin-treated Caco-2 cells than those in the untreated Caco-2 cells which suggested more expression of PEPT1. Conclusion The uptake of L-valyl-α-hederin is mediated by PEPT1.