反相高效液相色谱（RP-HPLC）中的流动相对组分具有很强的亲和力，并且与固定相共同竞争组分，流动相的种类及配比直接影响到组分的分离。因此，正确的选择流动相，确定最佳的色谱分离条件，可以实现更理想的分离。结合日常实验经验和相关文献，综述了在RP-HPLC中，如何快速地进行流动相的选择与优化。

The mobile phase of reversed phase high performance liquid chromatography (RP-HPLC) has a strong affinity for the components and competes for the components with the stationary phase. The species and proportion of the mobile phase directly affect the separation of components. Therefore, correctly choosing the mobile phase and determining the best chromatographic conditions could achieve better separation. Combining daily experiences with related literatures, the selection and optimization of mobile phase in RP-HPLC has been discussed.