目的 建立测定阿片碱在小鼠血浆中浓度的LC-MS/MS方法，并将该方法用于夏天无提取物在小鼠体内的药动学研究。方法 采用LC-MS/MS法。色谱条件采用安捷伦Zorbax C₁₈色谱柱（50 mm×2.1 mm，1.8 μm），流动相为甲醇-水（含0.1%甲酸），梯度洗脱；体积流量：0.2 mL/min；柱温：35 ℃；进样量：5 μL。质谱条件采用离子源：ESI源，正离子检测，高纯氮气用作干燥气（15 L/min）和气帘气（50 L/min），气体温度340 ℃；以多反应监测（multiple reaction monitoring，MRM）为扫描模式。采用回归方程计算小鼠血浆中阿片碱。小鼠ig给予夏天无提取物的羧甲基纤维素钠混悬液，制备血药质量浓度-时间曲线，计算药动学参数。结果 阿片碱在0.4～200.0 ng/mL线性关系良好，精密度RSD均小于15%，准确度在±15%以内，提取回收率在90%以上，基质效应在90%～110%。药动学参数最大血药浓度（C_max）和达峰时间（t_max）分别为134.6 ng/m、1.5 h。结论 该方法适合小鼠ig夏天无提取物后阿片碱在小鼠体内的药动学研究。

Objetive To establish an LC-MS/MS method for the determination of protopine in plasma of mice and appliy to in vivo pharmacokinetics study of Corydalis Decumbentis Rhizoma extract in mice. Methods LC-MS/MS method was performed. The HPLC analysis was carried out on an Angilent Zorbax C₁₈ column (50 mm × 2.1 mm, 1.8 μm). The mobile phase was methanol - water (0.1% formic acid water solution) with gradient elution. The column temperature was 35 ℃ with injection volume of 5 μL at a flow rate of 0.2 mL/min. MS conditions were that a triple-quadrupole mass spectrometry equipped with electrospray ionization (ESI) source for positive ion detection and multiple reaction monitoring (MRM) were applied. Dry gas (15 L/min) and air curtain gas (50 L/min) were used as high purity N₂ with temperature 340 ℃. The contents of protopine in in plasma samples of mice were determined with regression equation of standard curves. The blood concentration-time curve was investigated after ig administration of Corydalis Decumbentis Rhizoma extract suspension prepared with sodium carboxymethyl cellulose to mice, then the pharmacokinetic parameters were calculated. Results The method was linear over the concentration ranges of 0.4 - 200 ng/mL for protopine. The RSD values of intra-day and inter-day were less than 15%, the relative error (RE) were all within 15%, and the extract recovery was above 90%. The matrix effects were between 90% and 110%. The parameters of pharmacokinetics were C_max 134.6 ng/m, and t_max 1.5 h. Conclusion The method is suitable to in vivo pharmacokinetic study of protopine in mice after ig administered Corydalis Decumbentis Rhizoma extract.

国家自然科学基金资助项目（81360485）；江西省博士后科研择优资助项目（赣人社字[2012]195号）；江西中医药大学校级课题（ZX1002）