目的 探讨莱菔硫烷联合茶多酚对肺癌组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2的表达的影响。方法 选取经病理证实并手术治疗的肺癌患者56例，随机分为治疗组和对照组。采用ELISA测定组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2的浓度，免疫组化法测定组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2的表达并统计微血管密度（MVD），Western blotting法检测组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2蛋白表达情况。结果 肺癌患者组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2的浓度水平高于治疗组（P<0.05）；肺癌患者癌变组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2的表达均呈阳性，且对照组织中MVD高于治疗组（P<0.05），Western blotting法检测对照组组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2表达高于治疗组（P<0.05）。结论 蛋白激酶A锚定蛋白95、细胞周期蛋白E2在肺癌患者组织中均存在表达升高，与肿瘤生长呈正相关，莱菔硫烷联合茶多酚用药对两种蛋白起到抑制作用。

Objective To investigate the effect of sulforaphane combined with tea polyphenols on the expression of protein kinase A anchored protein 95 and cyclin E2 in lung cancer tissue. Methods The 56 cases confirmed by pathology and operation therapy for lung cancer patients were randomly divided into treatment group and control group. The levels of protein kinase A anchored protein 95 and cyclin E2 were determined by ELISA method. Protein A kinase anchoring protein 95, the expression of cyclin E2, and statistics of microvessel density (MVD) were determined by immunohistochemical method. The protein kinase A anchored protein 95 and cyclin E2 protein expression in tissues were detected by Western blotting method. Results The content of protein in the tissues of patients with lung cancer A kinase anchored protein 95 and cyclin E2 was higher than that of combination group, and the difference was statistically significant (P<0.05). The expression of cancerous tissue of lung cancer patients A kinase anchored protein 95 and cyclin E2 was positive, and the MVD was higher than that in control group (P<0.05). The cell protein kinase A anchored protein 95 and cyclin E2 protein in tissue in the control group were higher than those in treatment group by Western blotting (P<0.05). Conclusion The expression of cell protein kinase A anchored protein 95 and cyclin E2 increases in tissues of patients with lung cancer, which is positively correlated with cancer, and sulforaphane combined with tea polyphenols has inhibitory action.