目的 建立马齿苋药材中总多酚的测定方法，考察不同产地马齿苋药材中总多酚，为马齿苋药材质量评价提供参考。方法 以没食子酸为对照品，采用福林酚（FC）比色法测定马齿苋中总多酚。结果 不同产地的马齿苋药材中多酚含量在6.30～17.37 mg/g。结论 FC比色法简单快速，稳定，结果准确，适用于马齿苋中总多酚的测定；不同产地马齿苋中总多酚差异较大，建议马齿苋药材中总多酚质量分数以没食子酸计不低于6 mg/g。

Objective To establish a method for determination of total polyphenols in Portulaca oleracea, to investigate the total polyphenols in this herb from various habitats, and to provide reference for quality evaluation of P. oleracea. Methods The total polyphenols content in P. oleracea was determined by Folin-Ciocalteu (FC) colorimetric method with gallic acid as the standard. Results The total polyphenols of 11 batches of P. oleracea varied from 6.30 to 17.37 mg/g. Conclusion FC colorimetric method is a quick, easy-operating, precise, and stable method, which is suitable for the determination of total polyphenols in P. oleracea. In consideration of big differences of total polyphenols among P. oleracea from various habitats, it is suggested that the total polyphenols in P. oleracea crude drug should be above 6 mg/g.

国家自然科学基金面上项目（81073005）；山东大学自主创新项目（2012TS102）