目的 建立测定肌瘤化消颗粒中淫羊藿苷和丹参酮II_A的HPLC法。方法 采用高效液相色谱法，Diamonsil^TM C₁₈色谱柱（250 mm×4.6 mm，5 μm）；流动相：乙腈-水，梯度洗脱；检测波长为270 nm；柱温为40℃；体积流量为1.0 mL/min；进样量10 μL。结果 淫羊藿苷在7.5～50 μg、丹参酮II_A在3～20 μg与峰面积的线性良好（r=0.999 9）。平均回收率分别为100.2%、100.1%，RSD值分别为0.9%、1.3%（n=9）。结论 本法操作简便，重现性好，可有效控制肌瘤化消颗粒的质量。

Objective To establish an HPLC method for the determination of icariin and tanshinoneⅡ_A in Jiliu Huaxiao Granules. Methods HPLC was carried out on a Diamonsil^TM C₁₈ ( 200 mm×4.6 mm, 5 μm) column with acetonitrile-water as mobile phase for gradient elution. The detection wavelength was set at 270 nm. The injection volume was 10 μL at the flow rate of 1.0 mL/min. The temperature of column was set at 40℃. Results There was good linear relationship of icariin and tanshinoneⅡ_A in the range of 7.5-50 μg (r=0.999 9) and 3-20 μg (r=0.999 9), and the average recoveries were 100.2% and 100.1% with RSD values of 0.9% and 1.3%, respectively. Conclusion The method is simple to operate and reproducible, which can control the quality of Jiliu Huaxiao Granules effectively.