目的 利用HPLC-电雾式检测器（charged aerosol detector，CAD）联用技术建立知母中知母皂苷BⅡ和菝葜皂苷元的测定方法，并比较知母盐制前后两种成分的变化。方法 知母皂苷BⅡ以乙腈-水（25：75）为流动相，进样量为10 μL；菝葜皂苷元以甲醇-水（95：5）为流动相，进样量为20 μL；其他色谱条件均采用Thermo C₁₈柱（250 mm×4.6 mm，5 μm），体积流量为1 mL/min，柱温为30℃；Corona参数：氮气压力为241.3 kPa，Filter：High，Range：200 pA。结果 知母皂苷BⅡ在0.305～4.880 μg线性关系良好，r=0.999 4，平均回收率为99.1%，RSD值为1.2%；菝葜皂苷元在0.490～7.840 μg线性关系良好，r=0.999 1，平均回收率为98.0%，RSD值为1.3%。知母中知母皂苷BⅡ为3.16%～5.92%，盐制后为4.15%～7.20%；知母中菝葜皂苷元为0.42%～1.39%，盐制后为0.52%～1.54%。结论 CAD检测器具有较为平稳的基线和较高的灵敏度，更加适用于知母皂苷类及含有较弱或无紫外吸收成分的测定，知母盐制后知母皂苷BⅡ和菝葜皂苷元的量均有所升高。

Objective To establish a method for the determination of timosaponin BⅡ and sarsasapogenin in Anemarrhenae Rhizoma by HPLC with the charged aerosol detector (CAD), and to compare the contents of the two components in Anemarrhenae Rhizoma before and after processing with salt-water. Methods Analysis was performed on Thermo C₁₈ column (250 mm×4.6 mm, 5 μm). Acetonitrile-water (25:75) was as the mobile phase for timosaponin BⅡ with the injection volume of 10 μL, and methanol-water (95:5) as the mobile phase for sarsasapogenin with injection volume of 20 μL. The other chromatographic conditions were : flow rate of 1 mL/min and column temperature of 30℃. The Corona parameters were as follows: nitrogen pressure was 241.3 kPa, high filter, and range of 200 pA. Results The linear range of timosaponin BⅡ was 0.305-4.880 μg (r=0.999 4), and the average recovery rate was 99.1% (RSD=1.2%). The linear range of sarsasapogenin was 0.490-7.840 μg (r =0.999 1), and the average recovery rate was 98% (RSD=1.3%). The contents of timosaponin BII were obtained over 3.16%-5.92% for Anemarrhenae Rhizoma and 4.15%-7.20% after processing; The contents of sarsasapogenin were obtained over 0.42%-1.39% for Anemarrhenae Rhizoma and 0.52%-1.54% after processing. Conclusion CAD has some advantages in saponins detection of Anemarrhenae Rhizoma such as its steady baseline and good sensitivity. CAD is the most favorable detector for the determination of saponins or other constituents without UV absorption, and the contents of timosaponin BⅡ and sarsasapogenin increase after processing.

国家自然科学基金资助项目（81102810）；教育部高等学校博士学科点专项科研基金资助项目（20012133120009）