目的 测定硫磺熏前后黄芪中黄酮类和皂苷类成分的变化，确定硫磺熏对黄芪药材质量的影响，为评价熏硫加工对黄芪质量的影响提供依据。方法 对收集的黄芪进行熏硫加工，采用HPLC-DAD法测定了毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素4种黄酮成分，UPLC-ELSD法测定了黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪皂苷Ⅲ、黄芪甲苷4种皂苷成分。结果 硫磺熏过的黄芪药材中毛蕊异黄酮苷、芒柄花苷含量降低，毛蕊异黄酮、芒柄花素、黄芪皂苷Ⅰ、黄芪皂苷Ⅲ、黄芪甲苷的含量变化不明显。结论 黄芪熏硫加工后，黄酮苷成分有所降低，其余成分变化不明显。

Objective To analyze the contents of flavonoids and saponins in Astragali Radix before and after sulfur-fumigation, so as to provide reliable methods for scientifically evaluating and effectively controlling the quality of Astragali Radix. Methods After sulfur-fumigation, the contents of calycosin glucoside, ononin, calycosin, and formononetin were analyzed by HPLC-DAD, and astragaloside I, astragaloside Ⅱ, astragaloside Ⅲ, and astragaloside Ⅳ by UPLC-ELSD. Results The contents of calycosin glucoside and ononin decreased, while the contents of calycosin, formononetin, astragaloside I, astragaloside Ⅲ, and astragaloside Ⅳ were essentially the same. Conclusion After sulfur-fumigation, flavonoid glycoside components are reduced, and the remainder have no obvious change.

国家自然科学基金资助项目（81241137）；山西省留学人员科研资助项目（2010-53）