目的 建立淫羊藿Epimedium brevicornu中化学成分的超高压液相色谱–飞行时间质谱（UPLC/Q-TOF-MS）的分析方法。方法 Acquity UPLC BEH C18色谱柱（100 mm×2.1 mm，1.7 μm），流动相为0.1%甲酸水溶液–乙腈，梯度洗脱；体积流量为0.4 mL/min；柱温为45 ℃；进样量为2 μL。质谱检测采用正、负离子模式，电压分别为3.0、2.5 kV；离子源温度110 ℃；雾化温度400 ℃；雾化气体积流量800 L/h。结果 在10 min内完成淫羊藿中40个化学成分的鉴定。结论 建立了一种简单、快速、高效的UPLC/Q-TOF-MS方法对淫羊藿中化学成分进行了鉴定，为全面控制淫羊藿的质量和机理研究提供了基础。

Objective To establish a method for the analysis on the chemical constituents in Epimedii Herba by UPLC/Q-TOF-MS. Methods The method was performed on Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm), and the mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile with gradient elution program. The injection volume was 2 μL at the flow rate of 0.4 mL/min. The column temperature was 45 ℃. Both positive and negative modes were selected for qualitative analysis. The capillary voltages were set at 3.0 and 2.5 kV. The ion source temperature and atomization temperature were 110 and 400 ℃. The flow rate of nebulization gas was 800 L/h. Results The main peaks were separated clearly and respectively in 10 min, and 40 compounds were identified. Conclusion The established method for the determination of the chemical constituents in E. brevicornu using UPLC/Q-TOF-MS is simple, rapid, and reliable, and paves a way for the quality control and further study of Epimedii Herba.