目的 建立测定注射用银杏二萜内酯中银杏内酯A、B、C、K的HPLC-ELSD法。方法 采用HPLC-ELSD法，Phenomenex Luna C18色谱柱（250 mm×4.6 mm，5 μm），流动相为四氢呋喃–甲醇–水，梯度洗脱；体积流量为1.0 mL/min；柱温为30 ℃；漂移管温度为105 ℃，氮气体积流量为3.0 L/min。结果 注射用银杏二萜内酯中银杏内酯A、B、C、K分别在1.70～8.50 μg（r＝0.999 7）、2.45～12.5 μg（r＝0.999 8）、0.30～1.50 μg（r＝0.999 7）、0.25～1.25 μg（r＝0.999 7）呈良好的线性关系；平均回收率分别为101.03%、100.86%、100.78%、101.02%，RSD值分别为0.87%、0.29%、1.30%、1.34%。结论 该方法简便准确，重复性好，可用于注射用银杏二萜内酯中银杏内酯A、B、C、K的测定。

Objective To establish the method for determination of ginkgolide A, B, C, and K in Diterpene Lactones from Ginkgo biloba for injection by HPLC-ELSD. Methods HPLC-ELSD method was adopted. Phenomenex Luna C18 column (250 mm × 4.6 mm, 5 μm) was adopted with mobile phase consisted of tetrahydrofuran – methanol – water in gradient elution. The flow rate was 1.0 mL/min. The temperature of column and drift tube was set at 30 and 105 ℃, respectively. The flow rate of gas maintained at 3.0 L/min. Results The liner ranges of ginkgolide A, B, C, and K were 1.70 — 8.50 μg/mL (r=0.999 7), 2.45 — 12.5 μg/mL (r=0.999 8), 0.30 —1.50 μg/mL (r=0.999 7), and 0.25 — 1.25 μg/mL (r=0.999 7); The average recoveries were 101.03%, 100.86%, 100.78%, and 101.02%, with RSD values of 0.87%, 0.29%, 1.30%, and 1.34% (n=6), respectively. Conclusion The method could be used for the determination of ginkgolide A, B, C, and K in Diterpene Lactones from Ginkgo biloba for injection with a good sensitivity, accuracy, and reproducibility.

国家重大新药创制项目（2013ZX09402203）