目的 研究大孔树脂纯化两色金鸡菊总黄酮的工艺条件及参数。方法 采用静态吸附解吸法优选适宜的大孔树脂，以树脂吸附量、总黄酮解吸率为考察指标，对筛选出的树脂进行工艺优化。结果 D-101树脂具有较好的吸附及解吸附性能，其最佳工艺为：上样液质量浓度3.4 mg/mL，树脂的上样量为15 mL/g，上样液pH值为5.0，上样流速1.0 mL/min，6 BV去离子水洗脱除杂后，用pH 5.0的70%乙醇以1.5 mL/min洗脱5 BV；所得两色金鸡菊总黄酮质量分数为73.5%，得率为8.8%。结论 D-101树脂综合性能好，适合于两色金鸡菊总黄酮的分离纯化。

Objective To explore the technological parameters and the purification process of total flavonoids from Coreopsis tinctoria. Methods Nine types of macroporous resins were compared through static adsorption-desorption methods with adsorbability and desorption ratio as indexes to optimize the purification parameters. Results D-101 resin showed better adsorption and desorption parameters, and the optimal parameters for purification process were as follows: the concentration of sample extract was 3.4 mg/mL, the loaded amount was 15 mL/g, pH value was 5.0, the flow rate was 1.0 mL/min, and after being eluted with 6 BV water, the optimal solution for desorption was 70% ethanol in pH 5.0 with 5 BV at the flow rate of 1.5 mL/min. The purity of total flavonoids was 73.5%, and the yield was 8.8%. Conclusion D-101 resin shows significant effect on the purification of flavonoids which is feasible to separate and purify total flavonoids from C. tinctoria.

新疆维吾尔自治区重点实验室项目（XYSY0207-2009-01）；新疆留学回国人员科技活动择优资助项目（新人社函[2009]490号）