目的 建立茯苓皮中松苓新酸的HPLC测定方法。方法 采用高效液相色谱法，Lichrospher C18色谱柱（200 mm×4.6 mm，5 μm），流动相为乙腈–0.5%磷酸水溶液（80∶20），检测波长为242 nm，体积流量为1.0 mL/min，柱温为30 ℃，进样量为10 μL。结果 松苓新酸在0.142 5～2.280 0 μg与峰面积呈良好的线性关系（r=0.999 3），平均回收率为101.83%，RSD值为1.45%（n=6）。结论 该方法操作简便，稳定可行，重复性好，可用于茯苓皮中松苓新酸的测定。

Objective To establish an HPLC method for the determination of dehydrotrametenolic acid in the peels of Poria cocos. Methods HPLC was carried out on a Lichrospher C18 column (200 mm×4.6 mm, 5 μm) with acetonitrile-0.5% phosphoric acid (80∶20) as mobile phase. The detection wavelength was set at 242 nm. The injection volume was 10 μL at the flow rate of 1.0 mL/min. The column temperature was at 30 ℃. Results The calibration curve was linear over the range of 0.142 5—2.280 0 μg (r=0.999 3) for dehydrotrametenolic acid. The average recovery rate was 101.83% with RSD value at 1.45% (n=6). Conclusion The established method is rapid, sensitive, and accurate, and could be used for the determination of dehydrotrametenolic acid in the peels of P. cocos.