[关键词]
[摘要]
目的 考察枸杞提取液对球形红细菌生长的影响。方法 在不同质量浓度的常规培养基中添加不同质量浓度的枸杞提取液,培养球形红细菌,比较球形红细菌的活菌数、生长曲线和脱氢酶活性,考察枸杞提取液对球形红细菌生长的影响。结果 在培养基中添加枸杞提取液可缩短球形红细菌生长延迟期,使其提前进入指数期和稳定期;当质量浓度为12.5 g/L时,球形红细菌活菌数增加到之前的1.5倍,使球形红细菌脱氢酶活性提高到之前的3.3倍。结论 培养基中添加一定量的枸杞提取液可以促进球形红细菌的生长,使球形红细菌生长周期缩短、活力提高。
[Key word]
[Abstract]
Objective To study the effects of Lycium barbarum extract on the growth of Rhodobacter sphaeroides. Methods Adding L. barbarum extract into general culture medium at different concentration, the effects of L. barbarum extract on the growth of R. sphaeroides were investigated by culturing R. sphaeroide and comparing living R. sphaeroides bacteria number, growth curve, and dehydrogenase activity.. Results After adding L. barbarum extract into the culture medium, the growth delay period of R. sphaeroides was shortened and the expotential and stationary periods were advanced. When the concentration of L. barbarum extract was 12.5 g/L, the number of living R. sphaeroides was increased in 1.5 times, and the dehydrogenase activity of R. sphaeroides was improved in 3.3 times as much as before. Conclusion When L. barbarum extract is added into the culture medium, the growth and the activity of R. sphaeroides could be improved, and the growth cycle could be shortened.
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[基金项目]
国家自然科学基金资助项目(81173473);山西省自然科学基金资助项目(2010011048-2);太原市科技项目人才专项明星专题项目(120247-08);山西医科大学青年基金(02201128,02201124)