目的 研究热毒宁注射液及其组分对流感病毒神经氨酸酶（NA）抑制活性。方法 通过测定流感病毒NA的荧光强度值来确定热毒宁注射液及其组分对流感病毒NA的抑制活性。结果 热毒宁注射液对H1N1、H3N2、B流感病毒NA抑制活性较高，半数抑制浓度（IC50）分别为（46.49±2.25）、（49.77±1.77）、（45.33±5.32）μg/mL。金银花聚酰胺柱洗脱浓缩液、醋酸乙酯萃取液及残液、柱色谱提取物、隐绿原酸、异绿原酸A和异绿原酸B对H1N1、H3N2、B流感病毒NA酶活性均具有抑制作用。结论 热毒宁注射液及其有机酸类成分对流感病毒神经氨酸酶活性均具有一定抑制作用。 关键词：热毒宁注射液；流感病毒；神经氨酸酶

Objective To research the inhibitory activity of Reduning Injection and its components on neuraminidases (NA) in influenza virus. Methods The inhibition of Reduning Injection and its constituents on NA in influenza virus was determined by measuring the fluorescence intensity. Results Reduning Injection had strong inhibition on NA in H1N1, H3N2, and B influenza virus, and their half inhibitory concentration (IC50) values were (46.49 ± 2.25), (49.77 ± 1.77), and (45.33 ± 5.32) μg/mL. Lonicerae Japonicae Flos extract as polyamide column wash off concentrate, ethyl acetate extract and residue, column chromatography extracts, implicit chlorogenic acid, different chlorogenic acid A, and different chlorogenic acid B had inhibitory activities on NA in H1N1, H3N2, and B influenza virus. Conclusion Reduning Injection and the organic acid from Lonicerae Japonicae Flos have certain inhibitory activities on NA in influenza viruses.

国家重大新药创制项目（2013ZX09402203)