目的 研究硬棘软珊瑚醋酸乙酯部位的化学成分。方法 采用正、反相硅胶柱色谱以及半制备高效液相色谱进行分离，并根据理化性质和波谱数据鉴定化合物的结构。结果 分离得到11个化合物，其中3个环丁烯内酯类化合物，分别鉴定为(S)-13-(2-羟基-3,4-二甲基-5-氧代-2,5-二氢呋喃-2-基)十三烷酸（1）、(S)-11-(2-羟基-3,4-二甲基-5-氧代-2,5-二氢呋喃-2-基)十一烷酸（2）、羟基二氢博伏内酯（3）；1个嘌呤类化合物，鉴定为1,3-二甲基黄嘌呤（4）；7个甾体类化合物，分别鉴定为孕甾-20-烯-3-酮（5）、孕甾-1,4,20-三烯-3-酮（6）、3β-羟基-孕甾-20-烯（7）、3β-羟基-孕甾-5,20-二烯（8）、20-羟基-孕甾-1,4-二烯-3-酮（9）、20-羟基-孕甾-1-烯-3-酮（10）、17β-羟基-雄甾-1-烯-3-酮（11）。结论 环丁烯内酯类化合物是首次从该属软珊瑚中分离得到，其中化合物1和2为新化合物，而硬棘软珊瑚醋酸乙酯部位的化学成分主要为甾体类化合物。

Objective To investigate the chemical constituents in the ethyl acetate fraction from Scleronephthya sp.. Methods The compounds were isolated by column chromatography (silica gel, including reversed phase) and semi-prepative HPLC, and their structures were identified on the basis of physicochemical properties and spectroscopic data. Results Eleven compounds were separated, three of which were butenolides, identified as (S)-13-(2-hydroxy-3,4-dimethyl-5-oxo-2,5-dihydrofuran-2-yl) tridecanoic acid (1), (S)-11-(2-hydroxy-3,4-dimethyl-5-oxo-2,5-dihydrofuran-2-yl) undecanoic acid (2), and hydroxydihydrobovolide (3), one of which was purine, identified as 1,3-dimethylxanthine (4), seven of which were pregnane steroids, identified as pregna-20-en-3-one (5), pregna-1,4,20-trien-3-one (6), 3β-hydroxy-pregna-20-ene (7), 3β-hydroxy-pregna-5,20-diene (8), 20-hydroxy-pregna-1,4-dien- 3-one (9), 20-hydroxy-pregna-1-en-3-one (10), and 17β- hydroxy-androst-1-en-3-one (11). Conclusion Pregnane steroids are the main chemical constituents in ethyl acetate fraction of Scleronephthya sp., and compounds 1 and 2 are new compounds, while butenolides are obtained from Scleronephthya sp. for the first time.

国家高技术研究发展计划（863计划）项目（2011AA090701）