目的 建立肾上腺素、去甲肾上腺素原料药中有关物质的HPLC分析方法。方法 采用五氟苯基柱（250 mm×4.6 mm，5 μm），流动相为0.03 mol/L磷酸二氢铵–甲醇（98∶2），检测波长280 nm，进样量20 μL，体积流量1.0 mL/min，柱温20 ℃。结果 肾上腺素、去甲肾上腺素两种物质与其降解产物均能达到基线分离，重复性好，且呈现较好的线性关系。结论 该方法操作简单、快速、灵敏度高，可用于肾上腺素、去甲肾上腺素原料药中有关物质的测定。

Objective To establish an HPLC method for the determination of related substances in adrenaline and noradrenaline crude drugs. Methods The samples were performed on PFP column (250 mm × 4.6 mm, 5 μm) with the mobile phase of 0.03 mol/L ammonium dihydrogen phosphate-methanol (98∶2). The detection wavelength was 280 nm and column temperature was 20 ℃ with injection volume of 20 μL at a flow rate of 1.0 mL/min. Results Two components adrenaline, noradrenaline and their degradation products were well separated with good repeatability and linearity. Conclusion The method is simple, rapid, and sensitive, which could be applied for the determination of the related substances in adrenaline and noradrenaline crude drugs.