目的 采用高效液相色谱法建立胡黄连总苷的特征图谱。方法 Luna C18色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈–0.5%冰醋酸水溶液（15.5∶84.5），体积流量为1.0 mL/min，柱温为35 ℃，检测波长为275 nm，进样量为10 μL。结果 建立了胡黄连总苷的特征图谱及其技术参数，即供试品特征图谱中应有11个特征峰，参照物峰相应的峰为S峰，计算各特征峰与S峰的相对保留时间，其相对保留时间应在规定值的±5%之内。采用国家药典委员会《中药色谱指纹图谱相似度评价系2004A版》自动处理，10批胡黄连总苷的相似度均大于0.998。结论 本方法操作简便，准确可靠，可用于控制胡黄连总苷的内在质量。

Objective To establish the characteristic chromatograms for the identification of the total glucosides in Picrorhiza scrophulariiflora (TGPS). Methods Methods The column was Luna Cl8 column (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile - 0.5% glacial acetic acid (15.5: 84.5), at the flow rate of 1.0 mL/min. The detection wavelength was 275 nm. The column temperature was 35 ℃ with the injection volume of 10 μL. Results Characteristic chromatograms and technial parameters of the TGPS were established. Eleven common peaks were separated in the test sample, and S peak was the reference peak. The relative retention time of each characteristic peak and S peak were calculated, and their relative retention time should be within ± 5% of the specified value. The similarity indexes of 10 batches were calculated by software published by the Chinese Pharmacopeia Committee. All these indexes were more than 0.998. Conclusion The method is simple, accurate and can be used for the quality control of the TGPS.

国家科技支撑计划课题（2007BAI41B06）