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[摘要]
目的 建立HPLC-ELSD法同时测定杜仲中绿原酸和鹅掌楸苷的方法。方法 奥泰C18柱(250 mm×4.6 mm,5 μm);流动相:乙腈–0.2%醋酸水,梯度洗脱;体积流量:0.9 mL/min;柱温:35 ℃;漂移管105 ℃,气体流量:2.5 L/min。结果 绿原酸和鹅掌楸苷分别在0.021 4~4.280 0 μg和0.020 6~4.120 0 μg呈良好的线性关系;绿原酸和鹅掌楸苷紫的平均回收率分别为98.57%、103.37%,RSD值分别为2.23%、2.56%。结论 本法简便、准确,可用于杜仲中绿原酸和鹅掌楸苷的测定。
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[Abstract]
Objective To establish the method for the simultaneous determination of chlorogenic acid and liriodendrin in Eucommiae Cortex by HPLC-ELSD. Methods Alltima C18 column (250 mm×4.6 mm,5 μm) was used. The mobile phase consisted of acetonitrile - 0.2% acetic acid solution and the gradient elution was carried out. The flow rate was 0.9 mL/min. The temperature of column and drift tube was set at 35 ℃ and 105 ℃, respectively. The flow rate of gas maintained at 2.5 L/min. Results The linear ranges of chlorogenic acid and liriodendrin were 0.021 4 — 4.280 0 and 0.020 6 — 4.120 0 μg, respectively. The average recovery rates of chlorogenic acid and liriodendrin were 98.57% (RSD=2.23%) and 103.37% (RSD=2.56%). Conclusion The method is simple, convenient, and accurate, and could be used for the determination of chlorogenic acid and liriodendrin in Eucommiae Cortex.
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