目的 建立同时测定苍耳丸中连翘酯苷A和绿原酸的HPLC法。方法 采用HPLC法测定。色谱柱：Shim-pack VP-ODS（150 mm×4.6 mm，5 μm）；流动相：乙腈–0.2%磷酸溶液，梯度洗脱；体积流量：1.0 mL/min；检测波长：330 nm；进样量：10 μL；柱温：40 ℃。结果 连翘酯苷A在0.232～1.856 μg与峰面积积分值呈现良好的线性关系（r=0.999 6），平均回收率为97.82%，RSD值为1.55%；绿原酸在0.25～2.00 μg与峰面积积分值呈良好的线性关系（r=0.999 8），平均回收率为101.1%，RSD值为1.18%。结论 该方法简便，结果准确，重现性好，可作为苍耳丸的质量控制方法。

Objective To establish a method for the simultaneous determination of forsythoside A and chlorogenic acid in Canger Pill. Methods HPLC method was adopted. A Shim-pack VP-ODS C18 colum (150 mm × 4.6 mm, 5 μm) was adopted with mobile phase consisted of acetonitrile - 0.2% phosphoric acid solution in gradient elution. Detection wavelength was set at 330 nm. The flow rate was 1.0 mL/min. The injection volume was 10 μL at column temperature 40 ℃. Results The linear ranges of forsythoside A and chlorogenic acid were 0.232 — 1.865 μg (r=0.999 6) and 0.25 — 2.00 μg (r=0.999 8), respectively. The average recoveries were 97.82% (RSD=1.55%) and 101.1% (RSD=1.18%), respectively. Conclusion The method is simple, practicable, and accurate, which could be used for the quality control of Canger Pill.