目的 研究从小蓟药材中制备蒙花苷对照品的制备方法和鉴定技术。方法 采用石油醚脱脂、碱溶解酸沉淀法对70%乙醇小蓟药材提取物进行分离纯化，通过UV、1H-NMR和13C-NMR进行结构鉴定，TLC和HPLC法对产品进行纯度检测。结果 从小蓟中分离、纯化得到蒙花苷对照品。经TLC法检查无杂质斑点，HPLC法检测质量分数达97%。结论 该方法制备的蒙花苷对照品符合中药化学对照品的相关要求，可作为小蓟药材及其制剂质量控制以及中药药效物质基础研究用的化学对照品。

Objective To investigate the preparation method and identification technique of linarin reference substance from Cirsium setosum. Methods The 70% ethanol extract of C. setosum was isolated and purified with petroleum ether defatting, alkali dissolution and acid deposition. Structural identification was carried out by UV, 1H-NMR, and 13C-NMR, and purities were determined by TLC and HPLC methods. Results Linarin reference substances in C. setosum were isolated and purified. There were no impurity spots by TLC check, and the mass fraction of HPLC detection was up to 97%. Conclusion The linarin reference substance prepared by this method complies with the relevant requirements of the chemical markers in Chinese materia medica (CMM), and could be used as chemical reference substance of C. setosum and quality control for its preparations, as well as the study on the pharmacodynamic material basis of CMM.