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[摘要]
目的 研究香青兰滴丸对大鼠急性心肌缺血损伤的保护作用,并与现有制剂进行对比。方法 结扎大鼠冠状动脉左前降支致急性心肌缺血模型,术后每天给予香青兰滴丸1次,连续给药5 d,采用Ⅱ导心电图(ECGⅡ)测心肌损伤程度;心脏用红四氮唑(TTC)染色测定心肌梗死范围;试剂盒法测定血清中天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)和肌酸磷酸激酶同工酶(CK-MB)的活性。结果 4.8、9.6 g/kg香青兰滴丸可以显著改善大鼠心肌缺血损伤,减小心肌梗死范围,分别减少了30.3%(P<0.01)、53.3%(P<0.001),降低AST、LDH和CK-MB的活性。与等剂量益心巴迪然吉布亚颗粒比较,香青兰滴丸对心肌梗死大鼠的各项测定指标差异均无显著性。结论 香青兰滴丸对大鼠急性心肌缺血损伤具有一定的保护作用,其疗效与益心巴迪然吉布亚颗粒相近。
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[Abstract]
Objective To study the protection of Xiangqinglan Dripping Pill on acute myocardial ischemic injury and compare with existing formulations. Methods Ligation of the left anterior descending coronary to induce acute myocardial ischemia model; Xiangqinglan Dripping Pill was given to rats postoperative once daily for consecutive 5 d; Ⅱ lead electrocardiogram (ECG Ⅱ) was used to measure the degree of myocardial injury; red tetrazolium (TTC) staining was used to determine the myocardial infarct size of heart; Kit method was used for the determination of the activities of serum aspartate aminotransferase enzyme (AST), lactate dehydrogenase (LDH), and creatine kinase isoenzyme (CK-MB). Results Xiangqinglan Dripping Pill (4.8, 9.6 g/kg) could significantly improve the myocardial ischemic injury in rats, decrease the myocardial infarct size by 30.3 % (P<0.01), 53.3 % (P<0.001), respectively, and reduce the activities of AST, LDH, and CK-MB. Compared with the same doses of Yixin Badiranjibuya Granule, the above indicators showed no significant difference. Conclusion Xiangqinglan Dripping Pill has the certain protection on acute myocardial ischemic injury and its efficacy is similar with Yixin Badiranjibuya Granule.
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