目的 探讨英夫利西单抗对哮喘模型豚鼠抗肿瘤坏死因子-α（TNF-α）的影响。方法 豚鼠50只，雌雄各半，随机分为5组：对照组，模型组，英夫利西单抗低、中、高剂量（5、10、15 mg/kg）组，每组10只。模型组和英夫利西单抗各剂量组ip给予10%卵蛋白以致敏，对照组给予等量生理盐水；模型组和英夫利西单抗各剂量组再分两次给予2%卵蛋白溶液雾化吸入，对照组以等量生理盐水替代。每次激发前英夫利西单抗各剂量组ip相应剂量的英夫利西单抗，第二次激发后24 h处死动物，对比各组血清、组织匀浆和肺泡灌洗液中TNF-α水平。结果 模型组血清、组织匀浆、肺泡灌洗液中TNF-α浓度高于对照组（P＜0.05），而英夫利西单抗各剂量组血清、组织匀浆、肺泡灌洗液中TNF-α的浓度低于模型组（P＜0.05）。结论 TNF-α与豚鼠哮喘形成密切相关；使用英夫利西单抗可以减少豚鼠哮喘模型血清、组织匀浆、肺泡灌洗液中TNF-α的产生。

Objective To investigate the effect of Infliximab on TNF-α of guinea pigs asthma model. Methods Guinea pigs (50), half male and female, were randomly divided into five groups, control, model, low-, mid-, and high-dose Infliximab (5, 10, and 15 mg/kg) groups (n=10). Model and the Infliximab groups were ip administered with 10% ovalbumin to sensitivate, and the control group was given physiological saline. Model and Infliximab groups were atomizing inhalated twice with 2% ovalbumin solution, and the control group was given the same dose physiological saline. Infliximab groups were ip administered with appropriate doses of Infliximab before excitation each time, and animals were killed 24 h after the second excitation. TNF-α was detected in serum, tissue, and bronchoalveolar lavage fluid. Results TNF-α concentration in the serum, tissue homogenates, and bronchoalveolar lavage fluid in the model group was higher than that in the control group (P < 0.05). However, TNF-α concentration in the serum, tissue homogenates, and bronchoalveolar lavage fluid of the Infliximab group was lower than that in the model group (P <0.05). Conclusion TNF-α is related to the formation of the guinea pig asthma, and Infliximab could reduce TNF-α of serum, tissue homogenates, and bronchoalveolar lavage fluid in guinea pig model of asthma.