目的 研究芫花中醋酸乙酯部位化学成分。方法 分别采用聚酰胺柱色谱、硅胶柱色谱、Sephadex LH-20柱色谱等分离技术对芫花醋酸乙酯部位进行分离纯化，根据波谱数据和理化性质确定化合物的结构。结果 从芫花醋酸乙酯部位分离得到11个化合物，分别鉴定为松脂醇（1）、(?)-异落叶松脂素（2）、落叶松脂醇（3）、对羟基苯甲酸（4）、3,4-二羟基苯甲酸（5）、山柰酚-3-β-D-(6-O-反-p-香豆酰)葡萄糖苷（6）、山柰酚-3-β-D-(6-O-顺-p-香豆酰)葡萄糖苷（7）、芫花素（8）、3′-羟基芫花素（9）、芹菜素（10）、木犀草素（11）。结论 化合物2、5、7为首次从该植物中分离得到。

Objective To study the chemical constituents in the ethyl acetate fraction from Daphne genkwa. Methods The chemical constituents in the ethyl acetate fraction from D. genkwa were isolated and purified by chromatography on polyamide, silica gel, Sephadex LH-20 columns, and their structures were identified by their physicochemical characteristics and spectral features. Results Eleven compounds were isolated and identified as pinoresinol (1), (?)-isolariciresinol (2), lariciresinol (3), 4-hydroxybenzonic acid (4), 3,4-diohydroxybenzoic acid (5), kaempferol-3-β-D-(6-O-trans-p-coumaroyl)glucopyranoside (6), kaempferol-3-β-D-(6-O-cis-p-coumaroyl) glucopyranoside (7), genkwanin (8), 3′-hydroxygenkwanin (9), apigenin (10) and luteolin (11). Conclusion Compounds 2, 5 and 7 are all isolated from this plant for the first time.