目的 建立消肿接骨贴中芍药苷测定的方法。方法 采用高效液相色谱法外标法测定。采用Thermo C18色谱柱（250 mm×4.6 mm，5 μm）；流动相：乙腈–0.1%磷酸（12∶88）；体积流量：1.0 mL/min；检测波长：230 nm；柱温：25 ℃；进样量10 μL。结果 芍药苷进样量在0.124 8～1.248 0 μg呈良好的线性关系（r＝0.999 8），平均回收率为98.94%，RSD值为1.73%（n=6）。结论 本研究方法专属性强，灵敏度高，简便、准确、重现性好，可用于消肿接骨贴中芍药苷的测定。

Objective To establish a method for the determination of paeoniflorin in Xiaozhong Jiegu Plaster. Methods The content of paeoniflorin was determined by HPLC with external standard method. Thermo C18 column (250 mm × 4.6 mm, 5 μm) was used with acetonitrile-0.1% orthophosphoric acid (12∶88) as the mobile phase at the flow rate of 1.0 mL/min. The detection wavelength was set at 230 nm and the column temperature was 25 ℃. The injection volume was 10 μL. Results The linear range of paeoniflorin was 0.124 8 — 1.248 0 μg (r=0.999 8) with an average recovery of 98.94% (RSD=1.73%, n=6). Conclusion The established method is specific, sensitive, simple, accurate, reproducible, and suitable for the quality control of paeoniflorin in Xiaozhong Jiegu Plaster.