目的 采用高效液相色谱法测定加味逍遥丸中甘草酸的方法。方法 色谱条件：Kromasil C18柱（250 mm×4.6 mm，5 μm）；流动相：乙腈–0.017 mol/L磷酸（36︰64）；体积流量：1 mL/min；柱温：30 ℃；检测波长：250 nm。结果 甘草酸在0.084～0.500 μg线性关系良好，r＝0.999 9，回收率为98.5%，RSD为0.80%（n＝6）。结论 本法准确可靠，重现性好，可用于测定加味逍遥丸中甘草酸。

Objective To establish an HPLC method for the determination of glycyrrhizic acid in Jiawei Xiaoyao Pills. Methods The chromatographic separation was performed on Kromasil C18 column (250 mm × 4.6 mm, 5 μm), the mobile phase was acetonitrile-0.017 mol/L phosphoric acid (36∶64), the flow rate was 1 mL/min at 30 ℃, and the detection wavelength was 250 nm. Results The linearity for glycyrrhizic acid was in a range of 0.084—0.500 μg (r = 0.999 9), and the average recovery and RSD were 98.5% and 0.80%, respectively (n = 6). Conclusion The method is accurate, reliable, and reproducible, and could be used as a quality control method for the determination of glycyrrhizic acid in Jiawei Xiaoyao Pills.