[关键词]
[摘要]
目的 制备依托泊苷长循环亚微乳,并对其进行理化性质研究。方法 在依托泊苷亚微乳处方的基础上,通过考察乳粒表面固有水化层厚度与聚乙二醇2000–二硬脂酰磷脂酰乙醇胺(PEG 2000-DSPE)用量的关系,确定PEG 2000-DSPE用量,从而确定了依托泊苷长循环亚微乳的处方和制备工艺,并进行了理化性质研究。结果 依托泊苷长循环亚微乳的浓度为0.8 mg/mL,平均粒径为(185.4±5.7)nm,Zeta电位为(?51.6±0.8)mV,药物包封率为97.52%。结论 得到了包封率在95%以上的依托泊苷浓度为0.8 mg/mL的依托泊苷长循环亚微乳的处方和制备工艺。
[Key word]
[Abstract]
Objective To prepare etoposide long circulating submicroemulsion (ELCSE) and to study its physico-chemical properties. Methods Based on the formulation of etoposide submicroemulsion, the quantity of PEG 2000-DSPE was decided by studying the relationship between the fixed aqueous layer thickness (FALT) of emulsion drop surface and the quantity of PEG 2000-DSPE. Then the ELCSE formulation and the preparing technique were decided, and the physico-chemical properties were studied. Results The concentration of etoposide was 0.8 mg/mL. The mean particle size, Zeta potential, and encapsulation efficiency of ELCSE were (185.4 ± 5.7) nm, (?51.6 ± 0.8) mV, and 97.52%, respectively. Conclusion The ELCSE formulation and preparing technique with etoposide concentration of 0.8 mg/mL and encapsulation efficiency over 95% are obtained.
[中图分类号]
[基金项目]
国家重点基础研究发展计划(973计划)资助项目(2010CB735602,2012CB724002)