目的 合成一长链肽47肽。方法 采用Fmoc/t-Bu正交保护多肽固相合成策略，使用9-芴甲氧羰基（Fmoc）保护氨基酸的α-氨基，用Liberty微波多肽合成仪，每个耦合循环用20%哌啶脱除Fmoc保护基团，连续添加氨基酸，并用茚三酮法检测反应终点，有效检测反应的进度，最后用82.5%的三氟乙酸将多肽从树脂上切割下来，得到47肽粗品。经过反相高效液相制备系统纯化得到多肽纯品，用反相高效液相分析仪和傅里叶高分辨质谱仪验证该产物。结果 高分辨质谱仪验证了该产物，纯品质量分数达98%。结论 微波可促进多肽特别是长链肽的合成。

Objective To synthesize a long-chain peptide—47 peptide. Methods Fmoc/t-Bu orthogonal protection solid phase synthesis strategy was used to synthesize the long-chain peptide, using 9-fluorenylmethoxycarbonyl (Fmoc) to protect the alpha amino of amino acid. With Liberty Microwave Peptide Synthesizer, 20% piperidine was used to remove Fmoc for each coupling cycle, amino acids were continuously added, and ninhydrin was used to detect the end point of reactions. The reaction progress was effectively detected, and finally 82.5% trifluoroacetic acid was used to cut off the peptide from resin, getting crude peptides. Finally, the crude peptide was purified by preparative RP-HPLC. The peptide was analyzed by analytical RP-HPLC and Fourier high-resolution mass spectrometer. Results The product has been validated High Resolution Mass Spectrometer and the purity of peptide is 98%. Conclusion Microwave could promote the synthesis of polypeptide, especially long-chain peptide.

国家自然科学基金资助项目（2009ZX09301-008-P-05）；国家重大新药创制“十一五”科技重大专项（2011ZX09041-009）