目的 建立灵芝子实体和孢子粉中3种灵芝酸类成分的HPLC测定方法，为全面评价灵芝药材和孢子粉的质量提供依据。方法 采用HPLC方法测定，Diamonsil（钻石）C8色谱柱（250 mm×4.6 mm，5 μm），流动相乙腈–0.03%磷酸（28∶72），体积流量1.2 mL/min，检测波长250 nm；柱温35 ℃。结果 灵芝酸C2、灵芝酸G、灵芝酸A分别在0.40～10.06 μg（r=0.999 99）、0.40～10.00 μg（r=0.999 99）、0.40～10.00 μg（r=0.999 99）线性关系良好，平均回收率分别为101.04%、99.39%、101.22%，RSD值分别为1.64%、1.86%、2.20%（n=6）；灵芝子实体中3种灵芝酸的质量分数分别在0.06～0.29 mg/g、0.12～0.37 mg/g、0.19～0.41 mg/g，灵芝孢子粉供试品中3种灵芝酸的质量分数分别在0～0.01 mg/g、0、0～0.03 mg/g。结论 本方法简便，稳定，可用于灵芝三萜类成分C2、灵芝酸G、灵芝酸A的定量分析。

Objective To provide the scientific evidence for overall quality evaluation of Ganoderma, a trial to develop HPLC method for quantitative analysis of three ganoderic acids in Ganoderma was carried out. Methods The HPLC method was performed on Diamonsil C8 column (250 mm×4.6 mm, 5 μm), with acetonitrile-0.03% phospholic acid (28∶72) as the mobile phase; The flow rate was 1.2 mL/min; UV detection wavelength was 250 nm; The column temperature was 35 ℃. Results The liner ranges of ganoderic acid C2, ganoderic acid G and ganoderic acid A were 0.40—10.06 μg (r=0.999 99), 0.40—10.00 μg (r=0.999 99) and 0.40—10.00 μg (r=0.999 99); The average recoveries were 101.04%, 99.39% and 101.22%, with RSD values of 1.64%, 1.86%, and 2.20% (n=6), respectively. The content ranges of three ganoderic acids in sporophore were 0.06—0.29 mg/g, 0.12—0.37 mg/g, and 0.19—0.41 mg/g, respectively, and those in spore powder were 0—0.01 mg/g, 0, and 0—0.03 mg/g, respectively. Conclusion The method is proved to be convenient and reliable, so it could be used for the quantitative analysis of ganoderic acids in Ganoderma.

国家重大新药创制科技专项（2011ZX09401-009）