目的 建立HPLC法同时测定心脑络通颗粒中野黄芩苷和4,5-二-O-咖啡酰奎宁酸的方法。方法 色谱柱为岛津Interstil ODS柱（250 mm×4.6 mm，5 μm），流动相为乙腈–0.1%三氟乙酸（25∶75），体积流量1.0 mL/min，检测波长335 nm，柱温37 ℃，进样量10 μL。结果 2种成分均能达到基线分离，重现好，且呈现较好的线性关系；加样回收率分别为97.1%、99.8%，RSD值分别为0.66%、0.82%。结论 该方法准确、灵敏、可靠，重复性好，可用于心脑络通颗粒的快速检测。

Objective To develop an HPLC method for simultaneous determination of scutellarin and 4,5-dicaffeoylquinic acid in Xinnao Luotong Granules. Methods The samples were separated on Shimadzu Interstil ODS column (250 mm×4.6 mm, 5 μm) with acetonitrile-0.1% trifluoroacetic acid (25∶75) as mobile phase. The detection wavelength was 335 nm and column temperature was 37 ℃ with injection volume of 10 μL at a flow rate of 0.8 mL/min. Results Two active components were well separated with good repeatability and linearity. The calibration curves were linear. The average recovery rates were 97.1% and 99.8% with RSD values at 0.66% and 0.82%, respectively. Conclusion The method is accurate, sensitive, credible, and repeatable, and could be used for the rapid detection of Xinnao Luotong Granules.