目的 建立女金糖浆中橙皮苷、黄芩苷和丹皮酚的HPLC分析方法。方法 采用资生堂Spolar-C18色谱柱（250 mm×4.6 mm，5 μm）；流动相为[甲醇–乙腈–0.1%磷酸（12∶13∶75）]–乙腈，进行梯度洗脱；柱温40 ℃；体积流量1.0 mL/min；检测波长283 nm；进样量10 μL。结果 橙皮苷、黄芩苷和丹皮酚分别在0.073 95～2.218 49、0.508 20～10.164 00、0.158 71～2.380 60 μg线性关系良好，回收率分别为97.0%、98.9%、99.0%，RSD值分别为0.33%、0.41%、0.14%（n=6）。结论 该方法方便、快速、灵敏度高，可以为女金糖浆的质量控制提供依据。

Objective To establish an HPLC method for the determination of hesperidin, baicalin, and paeonol in Nüjin Syrup. Methods The samples were separated on Shiseido Spolar-C18 column (250 mm×4.6 mm, 5 μm) with [methanol-acetonitrile-0.1% phosphoric acid (12∶13∶75)]-acetonitrile as mobile phase by gradient elution. The detection wavelength was 283 nm and column temperature was 40 ℃ with injection volume of 10 μL at a flow rate of 1.0 mL/min. Results The calibration curves were linear over the range of 0.073 95—2.218 49 μg, 0.508 20—10.164 00, and 0.158 71—2.380 60 μg (r=0.999 1) for hesperidin, baicalin and paeonol. The average recovery rates were 97.0%, 98.9%, and 99.0% with RSD at 0.33%, 0.41%, and 0.14%, respectively (n=6). Conclusion This method is simple, rapid, and sensitive, and could provide the basis for the quality control of Nüjin Syrup.