[关键词]
[摘要]
目的 研究芙蓉菊多糖的体外抗氧化活性。方法 采用1,1-二苯基苦基苯肼(DPPH)自由基、还原力、超氧阴离子、羟自由基4种体外抗氧化模型研究芙蓉菊多糖的抗氧化活性,用维生素C作对照。结果 芙蓉菊粗多糖对DPPH自由基、还原力、超氧阴离子、羟自由基均有明显的清除能力,其中DPPH、超氧阴离子、羟自由基的EC50值分别为0.273、0.669、0.594 mg/mL,对羟自由基清除能力强于维生素C。芙蓉菊多糖对自由基清除率与其质量浓度存在着明显的量效关系。结论 芙蓉菊多糖具有良好的体外抗氧化活性,作为天然抗氧化剂和自由基清除剂有进一步研究的价值。
[Key word]
[Abstract]
Objective To study the in vitro antioxidative activity of Crossostephium chinense polysaccharide (CCPS). Methods Four models of in vitro anti-oxidative activity were used to study, including DPPH, reducing power, superoxide anion radicals, and hydroxyl free radicals, compared with vitamin C. Results CCPS had the evident free radical scavenging capacities on DPPH, reducing power, superoxide anion radicals, and hydroxyl free radicals, and their EC50 values on DPPH, superoxide anion radicals, and hydroxyl free radicals were 0.296, 0.669, and 0.594 mg/mL, respectively. Hydroxyl scavenging capacity of CCPS was higher than that of vitamin C. There was a good linear relationship between the concentration and free radical scavenging capacity of CCPS. Conclusion CCPS has a good in vitro anti-oxidative activity and it deserves to be further studied as a natural anti-oxidant and free radical scavenger.
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[基金项目]
国家星火计划项目(2012GA700194)